Magnetic reconnection is a ubiquitous process that drives global-scale dynamics in plasmas. For reconnection to proceed, both ion and electrons must be unfrozen in a localized diffusion region. By analyzing in situ measurements, we show that the non-gyrotropic ion pressure is mainly responsible for breaking the ion frozen-in condition in reconnection. The reported non-gyrotropic ion pressure tensor can specify the reconnection electric field that controls how quickly reconnection proceeds.